Richard Greene

Sir Henry Baskerville

Basil Rathbone

Sherlock Holmes

Wendy Barrie

Beryl Stapleton

Nigel Bruce

Doctor John H. Watson

Lionel Atwill

Dr. James Mortimer

John Carradine

Barryman

Barlowe Borland

Frankland

Beryl Mercer

Mrs. Jennifer Mortimer

Morton Lowry

John 'Jack' Stapleton

Ralph Forbes

Sir Hugo Baskerville

E. E. Clive

London Cabbie John Clayton

Eily Malyon

Mrs. Barryman

Nigel De Brulier

The Notting Hill Murderer

Mary Gordon

Mrs. Hudson

Ruth Terry

Betsy Ann

Mary Young

Betsy Ann's Mother (uncredited)

Peter Willes

Roderick

Leonard Carey

Hugo's Servant (uncredited)

Rita Page

Chambermaid (uncredited)

Frank McLure

Ship Passenger (uncredited)