Saoirse Ronan

Mary Stuart

Margot Robbie

Queen Elizabeth I

Jack Lowden

Henry Darnley

Joe Alwyn

Robert Dudley

David Tennant

John Knox

Guy Pearce

William Cecil

Gemma Chan

Bess of Hardwick

Martin Compston

Earl of Bothwell

Ismael Cruz Cordova

David Rizzio

Brendan Coyle

Earl of Lennox

Ian Hart

Lord Maitland

James McArdle

James, Earl of Moray

Richard Cant

Thomas Andrews

Guy Rhys

Earl of Kent

Thom Petty

Earl of Shrewsbury

Izuka Hoyle

Mary Seton

John Ramm

Bull

Simon Russell Beale

Robert Beale

Maria Dragus

Mary Fleming

Liah O'Prey

Mary Livingston

Eileen O'Higgins

Mary Beaton

Greg Miller Burns

Thomas Hepburn

Aneurin Pascoe

John Hepburn

Adrian Derrick-Palmer

George Dalgleish

Kal Sabir

The Laird of Ramorny

Luke Kidd

James Haliburton

Adrian Lester

Lord Randolph

Georgia Burnell

Kate Carey

Alan Turkington

Thomas Jenye

Euan Macnaughton

William Kirkcaldy of Grange

Scot Greenan

Hector Maclean

Karen Dunbar

Commoner

Ian Hallard

Sir Richard Knightley

Andrew Rothney

King James I

Angela Bain

Snuffer Woman

Abby Cassidy

Fotheringhay Gentlewoman

Shalisha James-Davis

Fotheringhay Gentlewoman

Adam Bond

Sir William Douglas

Katharine O'Donnelly

Young Holyrood Servant

Grace Molony

Dorothy Stafford

Luke Hobson

Henry Killigrew

Ben Wiggins

John Tamworth

Eldredd Wolf

William Killigrew

Eric MacLennan

Portrait Painter

Nathen East

Andrew Ker of Fawdonside

Daniel Booroff

Knox's Secretary

Sean Buchanan

Francis Yaxley

Matthew Pidgeon

Wedding Announcer

Benjamin Barraclough

Officer

Jordan Turk

James MacDonald

Adam Stevenson

Urie Campbell

Scot Greenan

Hector MacLean

Chris Grahamson

Moray's Drummer

Ed Jones

William Taylor

Owen Gorman

Bishop

John Stahl

Fisherman

Kadiff Kirwan

Attaché

Alex Beckett

Sir Walter Mildmay

Will Rowlands

Soldier

Grant Crookes

Bothwell

Philip Gascoyne

English Nobleman (uncredited)

Daniel Joseph Woolf

Queen's Privy Council (uncredited)