Shameik Moore

Miles Morales (voice)

Hailee Steinfeld

Gwen Stacy (voice)

Brian Tyree Henry

Jeff Morales (voice)

Luna Lauren Velez

Rio Morales (voice)

Jake Johnson

Peter B. Parker (voice)

Oscar Isaac

Miguel O'Hara (voice)

Jason Schwartzman

Spot (voice)

Issa Rae

Jessica Drew (voice)

Daniel Kaluuya

Hobie Brown (voice)

Karan Soni

Pavitr Prabhakar (voice)

Shea Whigham

George Stacy (voice)

Greta Lee

LYLA (voice)

Mahershala Ali

Uncle Aaron (voice)

Amandla Stenberg

Margo Kess: Spider-Byte (voice)

Jharrel Jerome

Miles G. Morales (voice)

Andy Samberg

Ben Reilly (voice)

Jack Quaid

Peter Parker (voice)

Rachel Dratch

Ms. Weber (voice)

Ziggy Marley

Lenny (voice)

Jorma Taccone

Adriano Tumino / '67 (voice)

J.K. Simmons

J. Jonah Jameson (voice)

Donald Glover

Aaron Davis

Elizabeth Perkins

May / Quippy Spider-Person (voice)

Kathryn Hahn

Doc Ock (voice)

Ayo Edebiri

Glory (voice)

Atsuko Okatsuka

Yuri (voice)

Peter Sohn

Ganke (voice)

Melissa Sturm

Mary Jane (voice)

Lorraine Velez

Maria (voice)

Nic Novicki

Lego Spider-Man (voice)

Taran Killam

Web-Slinger (voice)

Metro Boomin

Metro Spider-Man (voice)

Josh Keaton

Spectacular Spider-Man (voice)

Sofia Barclay

Malala Windsor: Spider-UK (voice)

Danielle Perez

Charlotte Webber: Sun-Spider (voice)

Yuri Lowenthal

Insomniac Spider-Man (voice)

Ismail Bashey

Additional Voices (voice)

Oscar Camacho

Additional Voices (voice)

David Michie

Additional Voices (voice)

Juan Pacheco

Additional Voices (voice)

Chrystee Pharris

Additional Voices (voice)

Ben Pronsky

Additional Voices (voice)

Al Rodrigo

Additional Voices (voice)

Stan Sellers

Additional Voices (voice)

Warren Sroka

Additional Voices (voice)

Deepti Gupta

Additional Voices (voice)

Sohm Kapila

Additional Voices (voice)

Christopher Miller

Additional Voices (voice)

Andrea Navedo

Additional Voices (voice)

Jacqueline Pinol

Additional Voices (voice)

Eliyas Qureshi

Additional Voices (voice)

Jaswant Dev Shrestha

Additional Voices (voice)

Jason Linere-White

Additional Voices (voice)

Sitara Attaie

Additional Voices (voice)

June Christopher

Additional Voices (voice)

Michelle Jubilee Gonzalez

Additional Voices (voice)

Marabina Jaimes

Additional Voices (voice)

Rez Kempton

Additional Voices (voice)

Lex Lang

Additional Voices (voice)

Phil Lord

Additional Voices (voice)

Richard Miro

Additional Voices (voice)

Shakira Ja'nai Paye

Additional Voices (voice)

Michelle Ruff

Additional Voices (voice)

Dennis Singletary

Additional Voices (voice)

Amanda Troop

Additional Voices (voice)

Ruth Zalduondo

Additional Voices (voice)

Kimberly Bailey

Additional Voices (voice)

Sanjay Chandani

Additional Voices (voice)

Jorge R. Gutierrez

Additional Voices (voice)

Miguel Jiron

Additional Voices (voice)

Luisa Leschin

Additional Voices (voice)

Caitlin McKenna

Additional Voices (voice)

Andrew Morgado

Additional Voices (voice)

Arthur Ortiz

Additional Voices (voice)

Juan Pope

Additional Voices (voice)

Mike Rianda

Additional Voices (voice)

Cedric L. Williams

Additional Voices (voice)

Kimiko Glenn

Peni Parker (voice) (uncredited)