Warner Baxter

Dr. Samuel Alexander Mudd

Gloria Stuart

Mrs. Peggy Mudd

Claude Gillingwater

Colonel Dyer

Arthur Byron

Mr. Erickson

O. P. Heggie

Dr. Mac Intyre

Harry Carey

Commandant

Francis Ford

Corporal O'Toole

John McGuire

Lieutenant Lovett

Francis McDonald

John Wilkes Booth

Douglas Wood

General Ewing

John Carradine

Sergeant Rankin

Fred Kohler Jr.

Sergeant Cooper

Ernest Whitman

Buck

Paul Fix

David Herold

Frank Shannon

Mr. Holt

Frank McGlynn Sr.

Abraham Lincoln

Etta McDaniel

Aunt Rosabelle

J.M. Kerrigan

Judge Maiben

Arthur Loft

Carpet Bagger

Paul McVey

General Hunter

Maurice Murphy

Orderly

Matthew Beard

Boy at Dr. Mudd's Door (uncredited)

Frank Baker

Undetermined Secondary Role (uncredited)

Stanley Blystone

Boy Seeking Dr. Mudd (uncredited)

Whitney Bourne

Undetermined Secondary Role (uncredited)

Lester Dorr

George A. Atzerodt (uncredited)

Robert Dudley

Druggist at Trial (uncredited)

Jan Duggan

Actress at Ford's Theatre (uncredited)

Dick Elliott

Actor at Ford's Theatre (uncredited)

Bess Flowers

Woman Sitting Behind Lincoln in Theatre Box (uncredited)

Bud Geary

Sergeant (uncredited)

Robert Homans

Sergeant (uncredited)

Paul Kruger

Soldier (uncredited)

Duke R. Lee

Sergeant (uncredited)

Wilfred Lucas

Testifying Colonel (uncredited)

Murdock MacQuarrie

Edman Spangler (uncredited)

James A. Marcus

Blacksmith (uncredited)

Eric Mayne

Court-Martial Participant (uncredited)

Paul McAllister

Doctor (uncredited)

J.P. McGowan

Ship's Captain (uncredited)

Wedgwood Nowell

Court-Martial Member (uncredited)

Jack Pennick

Corporal (uncredited)

George H. Reed

Black Man Giving Booth Directions (uncredited)

Paul Stanton

Agitating Orator (uncredited)

Tom Steele

Trooper (uncredited)

Harry Strang

Ship's Mate (uncredited)

Ray Turner

Black Soldier at Prison (uncredited)

Blue Washington

Black Soldier at Prison (uncredited)

Charles West

Washington (DC) Citizen (uncredited)

Lloyd Whitlock

Maj. Rathbone (uncredited)