Bob Hoskins

Eddie Valiant

Christopher Lloyd

Judge Doom

Joanna Cassidy

Dolores

Charles Fleischer

Roger Rabbit / Benny the Cab / Greasy / Psycho (voice)

Stubby Kaye

Marvin Acme

Alan Tilvern

R.K. Maroon

Richard LeParmentier

Lt. Santino

Lou Hirsch

Baby Herman (voice)

Betsy Brantley

Jessica's Performance Model

Richard Ridings

Angelo

Mike Edmonds

Stretch

Morgan Deare

Editor / Gorilla (voice)

Billy J. Mitchell

Forensic #2

Ed Herlihy

Newscaster

James O'Connell

Conductor

April Winchell

Mrs. Herman (voice)

Mae Questel

Betty Boop (voice)

Mel Blanc

Daffy Duck / Tweety / Bugs Bunny / Sylvester / Porky Pig (voice)

Tony Anselmo

Donald Duck (voice)

Joe Alaskey

Yosemite Sam / Foghorn Leghorn (voice)

Fred Newman

Stupid (voice)

David L. Lander

Smart Ass (voice)

June Foray

Wheezy / Lena Hyena (voice)

Wayne Allwine

Mickey Mouse (voice)

Russi Taylor

Minnie Mouse / Birds (voice)

Richard Williams

Droopy (voice)

Pat Buttram

Bullet #1 (voice)

Jim Cummings

Bullet #2 (voice)

Jim Gallant

Bullet #3 (voice)

Frank Sinatra

Singing Sword (voice) (archive sound)

Tony Pope

Goofy / Big Bad Wolf (voice)

Cherry Davis

Woody Woodpecker (voice)

Kathleen Turner

Jessica Rabbit (voice) (uncredited)

Jack Angel

Additional Voices (voice) (uncredited)

Nancy Cartwright

Dipped Shoe (voice) (uncredited)

Mickie McGowan

Mooing Cow / Flying Witch (voice) (uncredited)

Amy Irving

Jessica Rabbit (singing voice) (uncredited)

Frank Welker

Dumbo (voice) (uncredited)