Zach Tyler Eisen

Lucas Nickle (voice)

Julia Roberts

Hova (voice)

Meryl Streep

Queen (voice)

Nicolas Cage

Zoc (voice)

Paul Giamatti

Stan Beals (voice)

Bruce Campbell

Fugax (voice)

Regina King

Kreela (voice)

Lily Tomlin

Mommo (voice)

Larry Miller

Fred Nickle (voice)

Cheri Oteri

Doreen Nickle (voice)

Ricardo Montalban

Head of Council (voice)

Myles Jeffrey

Steve (voice)

Creagen Dow

Mullet Boy (voice)

Frank Welker

Spindle / Frog / Caterpillar (voice)

Mark DeCarlo

Fly (voice)

Jake T. Austin

Nicky (voice)

Allison Mack

Tiffany Nickle (voice)

Paul Greenberg

Sleeping Ant #1 / Head Lice #1 / Head Lice #2 / Head Lice #3 / Head Lice #4 / Brett (voice)

Rob Paulsen

Beetle (voice)

Nicole Sullivan

Ant #9 (voice)

Don Frye

Soldier Ant (voice)

Larry Cedar

Ant #8 (voice)

David Kaye

Sleeping Ant #2 / Guard Ant #3 / Wrangler Ant (voice)

Denzel Whitaker

Albert (voice)

Jake Goldberg

Helmet Boy / Nob / Red Teammate #1 (voice)

John A. Davis

Ant #19 (voice)

Tom Kenny

Drone Ant / Ant #2 / Ant #6 (voice)

Nika Futterman

Ant #1 / Ant #7 (voice)

Paul Rugg

Ant #5 (voice)

Candi Milo

Nurse Ant #3 (voice)

Pat Fraley

Ant Council #1 (voice)

Tyler James Williams

Blue Teammate #1 (voice)

Jaishon Fisher

Blue Teammate #4 (voice)

Max Burkholder

Blue Teammate #6 (voice)

Colin Ford

Red Teammate #4 (voice)

Kendall Ryan Sanders

Red Teammate #5 (voice)

Shane Baumel

Red Teammate #6 (voice)

Neil Ross

Wasp #1 / Wasp #5 (voice)

Bob Joles

Wasp #2 (voice)

Wally Wingert

Wasp #3 (voice)

Jonathan David Cook

Wasp #6 (voice)

Sean Donnellan

Soldier Ant #1 (voice)

Tress MacNeille

Old Council Ant (voice)

Clive Robertson

Hova's Wasp (voice)

Vernee Watson

Head Nurse (voice)

Richard Green

Wasp Leader (voice)