Shameik Moore

Miles Morales (voice)

Hailee Steinfeld

Gwen Stacy (voice)

Brian Tyree Henry

Jeff Morales (voice)

Luna Lauren Velez

Rio Morales (voice)

Oscar Isaac

Miguel O'Hara (voice)

Jake Johnson

Peter B. Parker (voice)

Jason Schwartzman

Spot (voice)

Issa Rae

Jessica Drew (voice)

Daniel Kaluuya

Hobie Brown (voice)

Nicolas Cage

Spider-Man Noir (voice)

John Mulaney

Spider-Ham (voice)

Kimiko Glenn

Peni Parker (voice)

Amandla Stenberg

Margo Kess (voice)

Karan Soni

Pavitr Prabhakar (voice)

Mahershala Ali

Uncle Aaron (voice)

Jharrel Jerome

Miles G. Morales (voice)

Greta Lee

LYLA (voice)

Andy Samberg

Ben Reilly (voice)

Shea Whigham

George Stacy (voice)